Defrance T, Vanbervliet B, Durand I, Briolay J, Banchereau J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Eur J Immunol. 1992 Nov;22(11):2831-9. doi: 10.1002/eji.1830221112.
The pan-T cell antigen CD5 has been shown to delineate two different mouse B cell subsets, originating from distinct progenitors. In man, on average, 30% of the tonsillar B cell pool expresses this antigen. In the present report, a detailed comparison of the CD5+ and CD5- B cell response to cytokines, following activation via surface immunoglobulins (sIg) or CD40 antigen, was undertaken. CD5+ B cells were positively selected by panning or by sorting from tonsils. Two-color immunofluorescence analysis performed on tonsillar B cell populations showed that CD5+ B cells displayed most of the phenotypic features of mantle zone B cells. CD5+ B cells could be stimulated for DNA synthesis by mitogenic concentrations of Staphylococcus aureus, Cowan I strain (SAC), insolubilized anti-IgM antibodies, immobilized anti-CD40 antibodies and phorbol 12-myristate 13-acetate (PMA). The growth-response of small dense CD5- B cells to these T cell-independent mitogens was comparable to that of CD5+ B cells, whereas the low-density, in vivo-activated, CD5- B cells were only marginally stimulated by Ig-cross-linking agents and PMA. Following ligation of sIg, both B cell subsets proliferated essentially in response to interleukin (IL)-2 and IL-4. When used in co-stimulation with immobilized anti-CD40 antibodies, IL-4 promoted growth of CD5+ and CD5- B cells, whereas IL-2 displayed only moderate stimulatory effects. CD5+ and CD5- B cells differentiated into Ig-secreting cells when they were co-cultured with SAC or cross-linked anti-CD40 antibodies and IL-2. However, IgM constituted the major component of the Ig response of CD5+ B cells, whereas high levels of IgG were secreted by CD5- B cells.
全T细胞抗原CD5已被证明可区分源自不同祖细胞的两种不同的小鼠B细胞亚群。在人类中，平均30%的扁桃体B细胞库表达该抗原。在本报告中，对通过表面免疫球蛋白（sIg）或CD40抗原激活后，CD5 +和CD5 - B细胞对细胞因子的反应进行了详细比较。通过淘选或从扁桃体中分选来阳性选择CD5 + B细胞。对扁桃体B细胞群体进行的双色免疫荧光分析表明，CD5 + B细胞表现出套区B细胞的大多数表型特征。CD5 + B细胞可被促有丝分裂浓度的金黄色葡萄球菌考恩I株（SAC）、不溶性抗IgM抗体、固定化抗CD40抗体和佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA）刺激进行DNA合成。小而致密的CD5 - B细胞对这些T细胞非依赖性有丝分裂原的生长反应与CD5 + B细胞相当，而低密度的、体内活化的CD5 - B细胞仅被Ig交联剂和PMA轻微刺激。sIg连接后，两个B细胞亚群主要响应白细胞介素（IL）-2和IL-4而增殖。当与固定化抗CD40抗体共同刺激使用时，IL-4促进CD5 +和CD5 - B细胞的生长，而IL-2仅显示出中等刺激作用。当CD5 +和CD5 - B细胞与SAC或交联的抗CD40抗体和IL-2共培养时分化为分泌Ig的细胞。然而，IgM是CD5 + B细胞Ig反应的主要成分，而CD5 - B细胞分泌高水平的IgG。
