Shearer B H, Jenkinson H F, McMillan M D
Department of Oral Biology and Oral Pathology, University of Otago, Dunedin, New Zealand.
J Oral Pathol Med. 1994 Apr;23(4):149-55. doi: 10.1111/j.1600-0714.1994.tb01104.x.
The effects of four different hyperplastic agents and of the carcinogen DMBA on cytokeratin expression in hamster cheek pouch epithelia were compared. Reversible hyperplasia was produced by the application of either oil of turpentine, vitamin A or TPA. No hyperplastic changes were produced by application of EPP. Apart from the transient appearance of a 45 kDa cytokeratin in one group treated with vitamin A, the immunohistochemical staining patterns and immunoblot profiles of cytokeratins from cheek pouches treated with each of the hyperplastic agents were identical to controls. Following application of DMBA, the cytokeratins stained with increased intensity in the spinous and granular cell layers. This was associated with increased amounts of 42-56 kDa cytokeratins and decreased production of 62-75 kDa cytokeratins. Monoclonal antibody AE1 detected a 45 kDa cytokeratin in extracts of DMBA-treated epithelia that was not detected in untreated epithelial extracts. Monoclonal antibody AE3 detected an additional 54 kDa cytokeratin band in extracts of DMBA-treated epithelia. These cytokeratin changes were present in preneoplastic epithelia and maintained in neoplastic epithelia.
比较了四种不同增生剂及致癌物二甲基苯并蒽（DMBA）对仓鼠颊囊上皮细胞角蛋白表达的影响。通过涂抹松节油、维生素A或佛波酯（TPA）可产生可逆性增生。涂抹乙基亚硝基脲（EPP）未产生增生性变化。除了在一组用维生素A处理的仓鼠颊囊中短暂出现一种45 kDa的细胞角蛋白外，用每种增生剂处理的颊囊细胞角蛋白的免疫组化染色模式和免疫印迹图谱与对照组相同。涂抹DMBA后，棘层和颗粒层中细胞角蛋白的染色强度增加。这与42 - 56 kDa细胞角蛋白量的增加及62 - 75 kDa细胞角蛋白产生的减少有关。单克隆抗体AE1在DMBA处理的上皮提取物中检测到一种在未处理上皮提取物中未检测到的45 kDa细胞角蛋白。单克隆抗体AE3在DMBA处理的上皮提取物中检测到一条额外的54 kDa细胞角蛋白条带。这些细胞角蛋白变化存在于癌前上皮中，并在肿瘤上皮中持续存在。
