Baert J H, Veys R J, Ampe K
Interdisciplinary Research Center, Katholieke Universiteit Leuven, Belgium.
J Oral Pathol Med. 1996 Oct;25(9):497-501. doi: 10.1111/j.1600-0714.1996.tb00304.x.
The effect of sodium lauryl sulphate (SLS) on cytokeratin (CK) gene expression in hamster cheek pouch epithelium was studied with a hybridohistochemical technique. Using specific human anti-sense RNA probes, the plausible hamster mRNA counterparts for these human CK mRNAs were localized by detection of heterologous hybrids. In comparison with normal epithelium, the expression and distribution pattern of CK mRNAs in the hamster cheek pouch were obviously changed after application of SLS. There was a decreased expression of CK mRNAs in the hyperplastic basal layer, and increased expression in the hypertrophic granular layer. Strikingly, hybridization with the human CK 18 cRNA probe revealed an additionally expressed CK mRNA in the SLS-treated epithelium that was not found in the untreated epithelium. The present study indicates that cRNA probes for human CK mRNAs can be used successfully, not only to distinguish between different hamster CK mRNAs but also to investigate changes in CK gene expression upon the induction of non-neoplastic and neoplastic alterations in the hamster cheek pouch model. This may help elucidate the molecular changes involved in epithelial pathologies.
采用杂交组织化学技术研究了十二烷基硫酸钠（SLS）对仓鼠颊囊上皮细胞角蛋白（CK）基因表达的影响。使用特异性人反义RNA探针，通过检测异源杂交体定位这些人CK mRNA可能的仓鼠mRNA对应物。与正常上皮相比，应用SLS后仓鼠颊囊中CK mRNA的表达和分布模式明显改变。增生性基底层中CK mRNA表达降低，而肥厚性颗粒层中表达增加。引人注目的是，与人CK 18 cRNA探针杂交显示，在经SLS处理的上皮中存在一种在未处理上皮中未发现的额外表达的CK mRNA。本研究表明，人CK mRNA的cRNA探针不仅可以成功用于区分不同的仓鼠CK mRNA，还可用于研究仓鼠颊囊模型中非肿瘤性和肿瘤性改变诱导后CK基因表达的变化。这可能有助于阐明上皮病变中涉及的分子变化。
