Nylander K, Stenling R, Gustafsson H, Roos G
Department of Oral Pathology, University of Umeå, Sweden.
J Oral Pathol Med. 1994 Apr;23(4):190-2. doi: 10.1111/j.1600-0714.1994.tb01111.x.
In a comparison of flow cytometric DNA measurements on fresh and paraffin-embedded material from primary squamous cell carcinomas of the head and neck region, we discovered that previously undetected aneuploid clones could be detected by dual parameter analysis of cytokeratin and DNA applied to disintegrated cells from paraffin sections. Using this new approach the correlation coefficient between DNA-indices from fresh and paraffin-embedded material increased from 0.423 to 0.904.
在对头颈部原发性鳞状细胞癌的新鲜组织和石蜡包埋组织进行流式细胞术DNA测量的比较中，我们发现，通过对石蜡切片中破碎细胞应用细胞角蛋白和DNA的双参数分析，可以检测到先前未检测到的非整倍体克隆。使用这种新方法，新鲜组织和石蜡包埋组织的DNA指数之间的相关系数从0.423提高到了0.904。
