Berlinger N T, Malone B N, Kay N E
Department of Otolaryngology, University of Minnesota Hospital and Clinic, MN.
Arch Otolaryngol Head Neck Surg. 1987 Dec;113(12):1301-6. doi: 10.1001/archotol.1987.01860120047006.
Aberrations in chromosome number, ploidy abnormalities, have been associated with malignancy and are predictive of outcome. Automated flow cytometry has made DNA analysis applicable to many solid tumors. Analysis can be performed on fixed specimens, allowing archival retrieval. The techniques, however, are unique and must be individually tested for each tumor type. Presently, few studies have been applied to head and neck cancers. This series of flow cytometric DNA analyses compares the results of 17 fresh and fixed head and neck squamous cell carcinoma specimens. Aneuploidy was present to a significant degree (47%). The method produced interpretable results in 100% of cases, with 100% reproducibility. Fresh and fixed tumor specimens yielded comparable results 76% of the time and, in fact, interpretability of fixed specimens was superior. This series demonstrates a practical and accurate flow cytometric DNA assay for fixed squamous cell carcinoma specimens, facilitating rapid retrospective ploidy analysis.
染色体数目异常，即倍体异常，与恶性肿瘤相关，并可预测预后。自动化流式细胞术已使DNA分析适用于许多实体瘤。分析可在固定标本上进行，便于档案检索。然而，这些技术是独特的，必须针对每种肿瘤类型单独进行测试。目前，很少有研究应用于头颈癌。本系列流式细胞术DNA分析比较了17例新鲜和固定的头颈鳞状细胞癌标本的结果。非整倍体在很大程度上存在（47%）。该方法在100%的病例中产生了可解释的结果，重现性为100%。新鲜和固定的肿瘤标本在76%的时间内产生了可比的结果，事实上，固定标本的可解释性更好。本系列展示了一种实用且准确的针对固定鳞状细胞癌标本的流式细胞术DNA检测方法，便于快速进行回顾性倍体分析。
