大鼠脑内δ-阿片受体的分子克隆与表达
Molecular cloning and expression of a delta-opioid receptor from rat brain.
作者信息
Abood M E, Noel M A, Farnsworth J S, Tao Q
机构信息
Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond.
出版信息
J Neurosci Res. 1994 Apr 15;37(6):714-9. doi: 10.1002/jnr.490370605.
We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high-affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats.
我们已经分离并鉴定了一种大鼠δ-阿片受体。推导的氨基酸序列（372个氨基酸）与小鼠δ-阿片受体DOR-1非常相似。事实上，这两个物种之间97%的氨基酸残基是保守的，而93%的核酸残基是相同的。用大鼠DOR-1作为探针在大鼠皮层中检测到一个6kb的mRNA。当在COS细胞中表达时，该克隆显示出对δ-阿片类药物具有高亲和力的阿片结合选择性。大鼠δ-阿片受体cDNA克隆将是研究大鼠δ-阿片受体功能的有用工具。
Zotero 插件