Czerny C P, Waldmann R, Büttner M
Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München.
Tierarztl Prax. 1994 Jun;22(3):230-3.
Neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (PPV) were generated by immunizing BALB/c-mice with gradient-purified PPV Orf D-1701 or purified envelopes. Epitope specificity studies identified three distinct epitopes localized in the virus envelope. These antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. For a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, antigen capture ELISA, and polymerase-chain-reaction were used.
