Housawi F M, Roberts G M, Gilray J A, Pow I, Reid H W, Nettleton P F, Sumption K J, Hibma M H, Mercer A A
Moredun Research Institute, International Research Centre, Penicuik, Midlothian, U.K.
Arch Virol. 1998;143(12):2289-303. doi: 10.1007/s007050050461.
A panel of 27 mouse monoclonal antibodies (Mabs) was raised against orf virus. Sixteen of these Mabs reacted with a protein with a molecular mass of 65 kDa, 8 reacted with a protein with a molecular mass of 39 kDa and three remain uncharacterised. Reactivity of the Mabs with a library of recombinant vaccinia viruses expressing various regions of the NZ-2 orf virus genome identified the approximate positions of the genes encoding these 2 immunodominant orf virus proteins. The gene encoding the 39 kDa protein was identified and sequenced. The protein was detected in an envelope fraction of orf virus and was shown to be homologous to the envelope protein encoded by the H3L gene of vaccinia virus. The 65 kDa protein has not been fully chracterised, but the gene encoding it has been localised to a 10 kbp region of the orf virus genome. The Mabs were used to discriminate 4 parapoxviruses derived from sheep, 2 from cattle and 1 each from a seal and squirrel. Eighteen Mabs reacted with all 4 sheep viruses, 19 Mabs reacted with both cattle viruses, 6 recognised seal parapoxvirus and 2 recognised the squirrel parapoxvirus. Only one of the 27 Mabs reacted with all 8 parapoxviruses suggesting it recognises a conserved epitope within the genus.
制备了一组针对羊口疮病毒的27种小鼠单克隆抗体（Mab）。其中16种Mab与一种分子量为65 kDa的蛋白质发生反应，8种与一种分子量为39 kDa的蛋白质发生反应，3种尚未鉴定。这些Mab与表达NZ - 2羊口疮病毒基因组不同区域的重组痘苗病毒文库的反应，确定了编码这两种免疫显性羊口疮病毒蛋白的基因的大致位置。鉴定并测序了编码39 kDa蛋白质的基因。该蛋白质在羊口疮病毒的包膜组分中被检测到，并显示与痘苗病毒H3L基因编码的包膜蛋白同源。65 kDa的蛋白质尚未完全鉴定，但编码它的基因已定位到羊口疮病毒基因组的一个10 kbp区域。这些Mab用于区分4种源自绵羊的副痘病毒、2种源自牛的副痘病毒以及分别来自海豹和松鼠的各1种副痘病毒。18种Mab与所有4种绵羊病毒发生反应，19种Mab与两种牛病毒都发生反应，6种识别海豹副痘病毒，2种识别松鼠副痘病毒。27种Mab中只有1种与所有8种副痘病毒都发生反应，这表明它识别该属内的一个保守表位。
