Fujitani Y, Nakajima K, Kojima H, Nakae K, Takeda T, Hirano T
Division of Molecular Oncology, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1994 Jul 29;202(2):1181-7. doi: 10.1006/bbrc.1994.2053.
IL-6 signals activate an IL-6 response element in the junB promoter, JRE-IL6, in a Ras-independent manner. IL-6 rapidly induced a DNA-binding activity to the Ets binding site of JRE-IL6 (JEBS), one of necessary DNA motifs of the IL-6 response element. The IL-6-induced JEBS-binding activity was indistinguishable from those to acute phase response element (APRE) in both kinetics of induction and its DNA-binding specificity. Purified APRE binding factors (APRFs) from IL-6-stimulated rat liver were found to be composed of multiple Stat3-related proteins and Stat1. Moreover the purified APRFs specifically made a complex with JRE-IL6 with the same mobility as that observed in the crude extracts. These results indicate that an immediate early signal of IL-6 leading to activation of JRE-IL6 is mediated by STAT family transcription factors.
白细胞介素-6（IL-6）信号以不依赖Ras的方式激活junB启动子中的白细胞介素-6反应元件JRE-IL6。IL-6迅速诱导对JRE-IL6的Ets结合位点（JEBS）的DNA结合活性，JEBS是IL-6反应元件的必要DNA基序之一。在诱导动力学及其DNA结合特异性方面，IL-6诱导的JEBS结合活性与对急性期反应元件（APRE）的活性没有区别。从IL-6刺激的大鼠肝脏中纯化的APRE结合因子（APRFs）被发现由多种与Stat3相关的蛋白质和Stat1组成。此外，纯化的APRFs与JRE-IL6特异性形成复合物，其迁移率与在粗提物中观察到的相同。这些结果表明，导致JRE-IL6激活的IL-6即时早期信号是由STAT家族转录因子介导的。
