用丝裂霉素C和γ射线照射处理的B细胞、T4细胞和T8细胞中的微核及有丝分裂指数。

Micronuclei and mitotic index in B-, T4- and T8-cells treated with mitomycin C and gamma-irradiation.

作者信息

Holmén A, Karlsson A, Bratt I, Högstedt B

机构信息

Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.

出版信息

Mutat Res. 1994 Aug 1;309(1):93-9. doi: 10.1016/0027-5107(94)90046-9.

DOI:10.1016/0027-5107(94)90046-9

PMID:7519737

Abstract

Lymphocytes were treated in vitro with mitomycin C and gamma-radiation at different doses (0-250 nmol/l and 0-2 Gy, respectively). After incubation in RPMI 1640 medium and stimulation with phytohemagglutinin for 72 h, the lymphocyte subgroups T4 (CD4), T8 (CD8) and B (CD19) were separated by an immunomagnetic method and analyzed for the presence of micronuclei. With mitomycin C the highest levels were found in T4- and B-cells. When micronuclei were induced by irradiation the T4-cells showed the highest frequencies and the B-cells the lowest. The outcome of B-cells with gamma-irradiation was probably due to a pronounced cytotoxic reaction in this cell type, which could be measured as a decrease in mitotic index.

摘要

淋巴细胞在体外分别用不同剂量（分别为0 - 250 nmol/l和0 - 2 Gy）的丝裂霉素C和γ射线进行处理。在RPMI 1640培养基中孵育并用植物血凝素刺激72小时后，通过免疫磁珠法分离淋巴细胞亚群T4（CD4）、T8（CD8）和B（CD19），并分析微核的存在情况。使用丝裂霉素C时，在T4细胞和B细胞中发现微核水平最高。当通过辐射诱导微核时，T4细胞显示出最高频率，而B细胞显示出最低频率。γ射线照射B细胞的结果可能是由于这种细胞类型中明显的细胞毒性反应，这可以通过有丝分裂指数的降低来衡量。