Terasaki M, Slater N T, Fein A, Schmidek A, Reese T S
Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7510-4. doi: 10.1073/pnas.91.16.7510.
Purkinje neurons in rat cerebellar slices injected with an oil drop saturated with 1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate [DiIC16(3) or DiI] to label the endoplasmic reticulum were observed by confocal microscopy. DiI spread throughout the cell body and dendrites and into the axon. DiI spreading is due to diffusion in a continuous bilayer and is not due to membrane trafficking because it also spreads in fixed neurons. DiI stained such features of the endoplasmic reticulum as densities at branch points, reticular networks in the cell body and dendrites, nuclear envelope, spines, and aggregates formed during anoxia nuclear envelope, spines, and aggregates formed during anoxia in low extracellular Ca2+. In cultured rat hippocampal neurons, where optical conditions provide more detail, DiI labeled a clearly delineated network of endoplasmic reticulum in the cell body. We conclude that there is a continuous compartment of endoplasmic reticulum extending from the cell body throughout the dendrites. This compartment may coordinate and integrate neuronal functions.
通过共聚焦显微镜观察，向注射了用高氯酸1,1'-二十六烷基-3,3,3',3'-四甲基吲哚羰花青（DiIC16(3)或DiI）饱和的油滴的大鼠小脑切片中的浦肯野神经元，以标记内质网。DiI扩散到整个细胞体和树突，并进入轴突。DiI的扩散是由于在连续双分子层中的扩散，而不是由于膜运输，因为它也在固定的神经元中扩散。DiI染色了内质网的一些特征，如分支点处的密度、细胞体和树突中的网状网络、核膜、棘以及在低细胞外Ca2+缺氧期间形成的聚集体。在培养的大鼠海马神经元中，由于光学条件提供了更多细节，DiI标记了细胞体内内质网的一个清晰界定的网络。我们得出结论，存在一个从细胞体延伸到整个树突的连续内质网区室。这个区室可能协调和整合神经元功能。
