大鼠小脑浦肯野细胞胞体和树突中伴刀豆球蛋白A结合位点的可视化。
The visualization of concanavalin A binding sites in Purkinje cell somata and dendrites of rat cerebellum.
作者信息
Wood J G, McLaughlin B J, Barber R P
出版信息
J Cell Biol. 1974 Nov;63(2 Pt 1):541-9. doi: 10.1083/jcb.63.2.541.
Abstract
The localization of concanavalin A (Con A) binding sites in Purkinje cell somata and dendrites has been studied using a peroxidase labeling technique. In the somata, the nuclear, Golgi, and endoplasmic reticulum (ER) membranes are rich in Con A binding sites. The hypolemmal cisternae, which are continuous with the ER from the soma and throughout the dendritic tree of Purkinje cells, are also rich in Con A binding sites. Other cisternae seen in these dendrites do not bind detectable amounts of Con A. The results suggest that a cisternal system, rich in carbohydrate, may be continuous from the nuclear envelope to distal dendritic segments of Purkinje cells. Such a system could play a role in the movement of materials from Purkinje somata to dendrites.
摘要
利用过氧化物酶标记技术研究了伴刀豆球蛋白A（Con A）结合位点在浦肯野细胞胞体和树突中的定位。在胞体中，核膜、高尔基体膜和内质网（ER）膜富含Con A结合位点。从胞体到浦肯野细胞整个树突的内质网相连的皮下池也富含Con A结合位点。在这些树突中看到的其他池不结合可检测量的Con A。结果表明，富含碳水化合物的池系统可能从核膜延伸至浦肯野细胞的远端树突段。这样的系统可能在物质从浦肯野细胞胞体向树突的移动中发挥作用。
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