Sadek S, Unterman T G, Bell S C
Department of Obstetrics and Gynaecology, Medical School, University of Leicester, UK.
J Reprod Fertil. 1994 May;101(1):67-75. doi: 10.1530/jrf.0.1010067.
A monospecific antibody was used to determine the immunocytochemical localization of insulin-like growth factor binding protein 1 (IGFBP-1) in the rat uterus. Immunoreactive IGFBP-1 was first detected from day 5 of pregnancy in the luminal and glandular epithelium. However, immunoreactivity was most intense from day 6 in the glandular epithelium, where it was associated with apically located granules. Immunoreactive glands were located only in non-decidualized endometrium, which was limited at the implant site to a thin basal layer by growth of the antimesometrial decidua from day 7. However, glands and associated immunoreactive IGFBP-1 were prominent in the inter-implant sites until day 9, although they were detected throughout pregnancy. Similar reactivity was detected in the glands of the basal endometrium in deciduomata-bearing animals, but these decreased in number from day 7 of pseudopregnancy. No immunoreactivity was detected during the oestrous cycle but could be induced in ovariectomized animals by sequential oestradiol and oestradiol plus progesterone treatment. The observations were consistent with IGFBP-1 representing a secretory product of the glandular epithelium and could either play a role in development of the trophoblastic component of the conceptus during the pre-placental period of anti-mesometrial implantation or in the endometrium acting as an inhibitor of local IGF-I action and in either case by transporting IGF-I from the stromal to the glandular luminal environment.
使用单特异性抗体确定胰岛素样生长因子结合蛋白1（IGFBP-1）在大鼠子宫中的免疫细胞化学定位。妊娠第5天首次在腔上皮和腺上皮中检测到免疫反应性IGFBP-1。然而，从第6天开始，腺上皮中的免疫反应性最强，且与顶端的颗粒相关。免疫反应性腺体仅位于未蜕膜化的子宫内膜中，从第7天开始，子宫系膜对侧蜕膜的生长将植入部位的未蜕膜化子宫内膜限制为一层薄的基底层。然而，直到第9天，植入部位之间的腺体和相关的免疫反应性IGFBP-1都很突出，尽管在整个妊娠期都能检测到。在患有蜕膜瘤的动物的基底层子宫内膜腺体中也检测到类似的反应性，但从假孕第7天开始，这些腺体的数量减少。在发情周期中未检测到免疫反应性，但在去卵巢动物中，通过依次给予雌二醇和雌二醇加孕酮处理可诱导产生免疫反应性。这些观察结果与IGFBP-1是腺上皮的一种分泌产物一致，它可能在胎盘前子宫系膜对侧植入期的胚胎滋养层成分发育中起作用，或者在子宫内膜中作为局部IGF-I作用的抑制剂起作用，无论哪种情况，它都通过将IGF-I从基质转运到腺腔环境中发挥作用。
