Fenton J, Mummert J, Childers M
Clinical Laboratory, Crozer-Chester Medical Center, Upland, Pennsylvania.
J Anal Toxicol. 1994 May-Jun;18(3):159-64. doi: 10.1093/jat/18.3.159.
The specificity of a GC/MS assay of morphine and codeine was examined with particular regard to the potential interference due to hydromorphone and hydrocodone, compounds that cross-react extensively in opiate screening tests. A GC/MS method with solid-phase extraction and acetylation or trimethylsilylation was used. The equilibrium between keto and enol forms of the potential interferents was evaluated. Sodium borohydride was tried as a means for converting potential interferents into products that are more clearly separated from morphine and codeine. It was found that borohydride reduction proceeded easily and led to products that could be distinguished from morphine and codeine. Moreover, the quantitation of morphine in the presence of hydromorphone was improved if the borohydride step was included in the analysis.
研究了吗啡和可待因的气相色谱/质谱（GC/MS）测定法的特异性，特别关注了氢吗啡酮和氢可酮可能产生的干扰，这两种化合物在阿片类药物筛查试验中会发生广泛的交叉反应。采用了一种具有固相萃取和乙酰化或三甲基硅烷化的GC/MS方法。评估了潜在干扰物酮式和烯醇式之间的平衡。尝试使用硼氢化钠将潜在干扰物转化为与吗啡和可待因更易分离的产物。结果发现，硼氢化钠还原反应容易进行，并产生了可与吗啡和可待因区分的产物。此外，如果在分析中加入硼氢化钠步骤，在存在氢吗啡酮的情况下吗啡的定量分析会得到改善。
