采用气相色谱-质谱联用技术（GC-MS）对血液和尿液中的阿片类物质进行定量分析。

Quantitation of opioids in blood and urine using gas chromatography-mass spectrometry (GC-MS).

作者信息

Goldberger Bruce A, Chronister Chris W, Merves Michele L

机构信息

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.

出版信息

Methods Mol Biol. 2010;603:399-410. doi: 10.1007/978-1-60761-459-3_39.

DOI:10.1007/978-1-60761-459-3_39

PMID:20077092

Abstract

The opioid and 6-acetylmorphine assays utilize gas chromatography-mass spectrometry (GC-MS) for the analysis of morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted by mixed-mode solid phase extraction. The morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine extracts are derivatized with N-methyl-bis(trifluoroacetamide) (MBTFA) producing trifluoroacetyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.

摘要

阿片类药物和6-乙酰吗啡检测采用气相色谱-质谱联用仪（GC-MS）分析血液和尿液中的吗啡、可待因、氢吗啡酮、氢可酮和6-乙酰吗啡。样本用氘代内标进行强化，并构建五点校准曲线。样本通过混合模式固相萃取法进行提取。吗啡、可待因、氢吗啡酮、氢可酮和6-乙酰吗啡提取物用N-甲基双（三氟乙酰胺）（MBTFA）进行衍生化，生成三氟乙酰衍生物。最后，使用选择离子监测GC-MS对提取物进行分析。