Tsitsikov E N, Ramesh N, Geha R S
Division of Immunology, Children's Hospital, Boston, MA 02115.
Mol Immunol. 1994 Aug;31(12):895-900. doi: 10.1016/0161-5890(94)90009-4.
The mouse CD40 ligand (CD40L) gene was cloned, sequenced and characterized. DNA sequence analysis showed that the CD40L gene comprises five exons and four intervening introns, spread over 13-14 kb of genomic DNA. The putative site for initiation of mRNA transcription was identified at 67 bp upstream of the translation initiation (ATG) codon. The nucleotide sequence of the 5'-flanking region of this gene revealed the presence of several regulatory regions including a TATA-like box, an Sp1-like box and six potential NF-AT-like motifs. The 3'-untranslated region of the murine CD40L gene contained two ATTTA-elements which are thought to confer instability to the mRNA of many cytokines and two adjacent dinucleotide repeates, (CT)25 and (CA)45. These elements may play a role in the post-transcriptional regulation of CD40L gene expression.
对小鼠CD40配体（CD40L）基因进行了克隆、测序和特征分析。DNA序列分析表明，CD40L基因由5个外显子和4个间隔内含子组成，分布在13 - 14 kb的基因组DNA上。在翻译起始（ATG）密码子上游67 bp处确定了mRNA转录起始的推定位点。该基因5'侧翼区的核苷酸序列显示存在几个调控区，包括一个类TATA盒、一个类Sp1盒和六个潜在的类NF-AT基序。小鼠CD40L基因的3'非翻译区包含两个ATTTA元件，它们被认为会使许多细胞因子的mRNA不稳定，以及两个相邻的二核苷酸重复序列（CT）25和（CA）45。这些元件可能在CD40L基因表达的转录后调控中发挥作用。
