De Angeli S, Fandella A, Conconi M T, Anselmo G, Parnigotto P P
Laboratory of Cellular Cultures, Regional Hospital USSL, Treviso, Italy.
Prostate. 1994 Sep;25(3):117-24. doi: 10.1002/pros.2990250302.
This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer-aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 microM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation.
本研究探讨了苏拉明对源自人前列腺增生组织并在体外培养的U285细胞株生长和形态的影响。进行FRAME细胞毒性试验以评估苏拉明诱导的生长抑制作用。在接种时和24小时后将细胞暴露于苏拉明；在有和没有苏拉明的情况下加入中性红。使用连接到计算机辅助系统的光学显微镜和扫描电子显微镜研究苏拉明诱导的形态变化。生长抑制取决于药物浓度和暴露时间。此外，苏拉明对中性红摄取的影响是可逆的。1000微摩尔的苏拉明会使细胞变成球形，并且它们无法形成单层。我们的数据表明，在延迟期添加苏拉明会降低细胞增殖速率。
