[一种利用脂质体、单克隆抗体和补体测定抗体和抗原的高度灵敏的均相方法]

[A highly sensitive, homogeneous method for determining antibodies and antigens by using liposomes, monoclonal antibodies and complement].

作者信息

Skopinskaia S N, Tamulevich Ia A, Iarkov S P, Zlobin V N, Kalinin Iu T

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1993 Jan-Feb(1):77-82.

PMID:7520654

Abstract

A simple and sensitive method for the determination of Francisella tularensis antigen and antibodies to this antigen by means of the complement lysis of liposomes sensitized with F. tularensis lipopolysaccharide (LPS) is proposed. The possibility of participation of monoclonal antibodies (McAb) in the complement-dependent lysis of liposomes has been studied. The method permits the detection of 50-100 ng/ml of soluble LPS antigen in the presence of antiserum or McAb IgG F-B11-x and of 50-10 ng/ml of it in the presence of IgG F-B11-x and anti-IgG within 90 minutes.

摘要

提出了一种通过用土拉弗朗西斯菌脂多糖（LPS）致敏的脂质体补体裂解来测定土拉弗朗西斯菌抗原及针对该抗原的抗体的简单灵敏方法。研究了单克隆抗体（McAb）参与脂质体补体依赖性裂解的可能性。该方法能够在抗血清或McAb IgG F-B11-x存在的情况下，在90分钟内检测到50 - 100 ng/ml的可溶性LPS抗原，在IgG F-B11-x和抗IgG存在的情况下，能检测到50 - 10 ng/ml的该抗原。