Engel P, Gribben J G, Freeman G J, Zhou L J, Nozawa Y, Abe M, Nadler L M, Wakasa H, Tedder T F
Department of Immunology, Duke University Medical Center, Durham, NC 27710.
Blood. 1994 Sep 1;84(5):1402-7.
T-cell activation is initiated after T-cell receptor binding to antigen, but also requires interactions between costimulatory molecules expressed on antigen-presenting cells. An important costimulatory molecule expressed by monocytes and activated B lymphocytes has been recently identified and termed B7-2 or B70. Independently, a new Cluster of Differentiation was defined in the Fifth International Leukocyte Differentiation Antigen Workshop as CD86, a molecule predominantly expressed by monocytes and activated B lymphocytes. In this study, the two monoclonal antibodies that defined CD86, FUN-1 and BU-63, were shown to bind to cDNA transfected cells expressing B7-2/B70. The FUN-1 monoclonal antibody also completely blocked the costimulatory activity of B7-2/B70 in functional assays. Therefore, the serologically defined CD86 differentiation antigen is the B7-2/B70 molecule.
T细胞活化在T细胞受体与抗原结合后启动，但也需要抗原呈递细胞上表达的共刺激分子之间的相互作用。最近已鉴定出一种由单核细胞和活化B淋巴细胞表达的重要共刺激分子，并将其命名为B7-2或B70。另外，在第五届国际白细胞分化抗原研讨会上定义了一个新的分化簇为CD86，该分子主要由单核细胞和活化B淋巴细胞表达。在本研究中，定义CD86的两种单克隆抗体FUN-1和BU-63被证明可与表达B7-2/B70的cDNA转染细胞结合。FUN-1单克隆抗体在功能测定中也完全阻断了B7-2/B70的共刺激活性。因此，血清学定义的CD86分化抗原就是B7-2/B70分子。
