Chen J K, Tsai R J, Lin S S
Department of Physiology, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):243-8. doi: 10.1007/BF02632046.
Pterygium is a degenerative corneal limbal process and UV irradiation has been suggested as being a major environmental predisposing factor. The invasive nature of the fibroblasts associated with pterygia raises the question as to whether these cells are transformed. To test this hypothesis, we established fibroblast strains from autologous and heterologous pterygial and conjunctival specimens, respectively, from subjects between 40 to 50 yr of age, and compared their growth characteristics in culture. All pterygial fibroblast strains exhibited a reduced dependence on serum and exogenous growth factors for growth and reached a saturation population density that was threefold higher than conjunctival fibroblasts cultured under the same conditions. In addition, all pterygial fibroblast strains were able to form colonies in soft agar in 5% fetal bovine serum at a 6.0 to 7.5% efficiency. Under the same experimental conditions, none of the conjunctival fibroblast strains were able to grow. The results presented support the conclusion that pterygial fibroblasts have acquired many of the properties of the transformed phenotype.
翼状胬肉是一种角膜缘退行性病变，紫外线照射被认为是主要的环境诱发因素。翼状胬肉相关成纤维细胞的侵袭性引发了这些细胞是否发生转化的问题。为了验证这一假设，我们分别从40至50岁受试者的自体和异体翼状胬肉及结膜标本中建立了成纤维细胞系，并比较了它们在培养中的生长特性。所有翼状胬肉成纤维细胞系对血清和外源性生长因子的生长依赖性降低，达到的饱和种群密度比在相同条件下培养的结膜成纤维细胞高三倍。此外，所有翼状胬肉成纤维细胞系在含5%胎牛血清的软琼脂中能够以6.0%至7.5%的效率形成集落。在相同实验条件下，结膜成纤维细胞系均不能生长。所呈现的结果支持翼状胬肉成纤维细胞已获得许多转化表型特性这一结论。
