Hutter P, Karch F
Laboratoire de Génétique, Université de Genève, Switzerland.
Experientia. 1994 Aug 15;50(8):749-62. doi: 10.1007/BF01919377.
The X-linked gene Hmr in Drosophila melanogaster, when mutated, rescues otherwise inviable interspecific hybrids from crosses between D. melanogaster and any of its three most closely related species D. simulans, D. mauritiana and D. sechellia. DNA from the site of a breakpoint at the putative locus of the gene has been cloned, and results of transcription and sequence analyses are presented. Three distinct mRNAs are transcribed from this locus, two of which are abundantly expressed throughout life. A third transcript, which is larger but rarer, appears to be disrupted by at least one of the two known mutations of Hmr. The gene encodes a mitochondrial ADP/ATP translocator protein, which plays an essential role in maintaining metabolic energy. Analysis of several cDNAs suggested that the rescue of hybrids may be dependent on mutations in the variable 3' end region of this gene, affecting the level and/or the stability of the largest messenger RNA.
果蝇中与X染色体连锁的基因Hmr发生突变时，能挽救黑腹果蝇与其三个关系最密切的物种（拟果蝇、毛里求斯果蝇和塞舌尔果蝇）杂交产生的原本无法存活的种间杂种。该基因假定位点处断点的DNA已被克隆，并给出了转录和序列分析结果。从该位点转录出三种不同的mRNA，其中两种在整个生命周期中都大量表达。第三种转录本更大但更稀少，似乎被Hmr的两个已知突变中的至少一个破坏了。该基因编码一种线粒体ADP/ATP转位蛋白，它在维持代谢能量方面起着至关重要的作用。对几个cDNA的分析表明，杂种的挽救可能取决于该基因可变3'端区域的突变，影响最大信使RNA的水平和/或稳定性。
