Maeda A, Suzuki Y, Matsumoto M
Microbiol Immunol. 1978;22(12):775-84. doi: 10.1111/j.1348-0421.1978.tb00431.x.
Newcastle disease virus grown in embryonated eggs was separated and purified by sucrose density gradient centrifugation into two distinct type of particles, B and T, the former being normal virus particles with high activities of hemagglutination, hemolysis, neuraminidase and infectivity, the latter being non-infectious virus particles with low activities of hemolysis and neuraminidase but high hemagglutination activity. B and T particles were shown to share a common antigen by immunodiffusion test. T particles were deficient in viral RNA, since they contained only 13s RNA in a small amount, whereas B particles possessed a large amount of 57s RNA and a small amount of 13s RNA. T particles interfered with the multiplication of normal Newcastle disease virus in primary cultures of chick embryo cells.
通过蔗糖密度梯度离心法，将在鸡胚中培养的新城疫病毒分离纯化，得到两种不同类型的颗粒，即B型和T型。前者是具有高血凝、溶血、神经氨酸酶活性和感染性的正常病毒颗粒，后者是溶血和神经氨酸酶活性低但血凝活性高的非感染性病毒颗粒。免疫扩散试验表明，B型和T型颗粒具有共同抗原。T型颗粒缺乏病毒RNA，因为它们仅含有少量的13s RNA，而B型颗粒含有大量的57s RNA和少量的13s RNA。T型颗粒可干扰新城疫病毒在鸡胚细胞原代培养物中的增殖。
