艰难梭菌毒素A刺激离体大鼠胰腺腺泡分泌酶。

Clostridium difficile toxin A stimulates enzyme secretion from isolated rat pancreatic acini.

作者信息

Małecka-Panas E, Triadafilopoulos G, Tsukamoto H, Howitt G, Pothoulakis C, LaMont T

机构信息

Section of Gastroenterology, University of California, VA Med Ctr, Davis.

出版信息

Mater Med Pol. 1993 Jul-Dec;25(3-4):127-31.

PMID:7520960

Abstract

Although Cl difficile bacteremia and the presence of antibodies to toxin A (TxA) have been reported, little information is available at present on TxA effect on the functional properties of various visceral organs. We have, therefore, examined the in vitro effects of TxA on amylase and trypsin secretion from rat isolated pancreatic acini. Dispersed rat pancreatic acini were exposed for 60 min to different concentrations of highly purified TxA and the rate of amylase, trypsin and LDH release were monitored. Free cytosolic calcium release in pancreatic acini after toxin A (10(-10)M to 10(-8)M) treatment was measured with Fura-2/AM, Ca-indicator dye. TxA (10(-10) to 10(-8)M) increased significantly the rate of both the amylase and trypsin secretion without any membrane damage, with toxin A exerting its action via calcium dependent pathway as suggested by intracellular calcium release measured with Fura-2/AM.

摘要

虽然已有艰难梭菌菌血症及抗毒素A（TxA）抗体存在的报道，但目前关于TxA对各种内脏器官功能特性影响的信息却很少。因此，我们研究了TxA对大鼠离体胰腺腺泡淀粉酶和胰蛋白酶分泌的体外作用。将分散的大鼠胰腺腺泡暴露于不同浓度的高度纯化TxA中60分钟，并监测淀粉酶、胰蛋白酶和乳酸脱氢酶（LDH）的释放速率。用钙指示剂染料Fura-2/AM测量毒素A（10⁻¹⁰M至10⁻⁸M）处理后胰腺腺泡中游离胞质钙的释放。TxA（10⁻¹⁰至10⁻⁸M）显著提高了淀粉酶和胰蛋白酶的分泌速率，且未造成任何膜损伤，如用Fura-2/AM测量的细胞内钙释放所示，毒素A通过钙依赖途径发挥作用。