Autio K, Renzi L, Catalan J, Albrecht O E, Sorsa M
Institute of Occupational Health, Laboratory of Molecular and Cellular Toxicology, Helsinki, Finland.
Mutat Res. 1994 Sep 1;309(2):315-20. doi: 10.1016/0027-5107(94)90108-2.
Female CB6F1 mice and male Wistar rats were exposed to different concentrations of 1,3-butadiene (1,3-BD) by inhalation. Micronucleus tests using both peripheral blood erythrocytes and femoral marrow cells of these animals were performed. Cells were stained either using conventional acridine orange (AO) staining or supravitally using AO-coated slides. Dose-dependent increases in the frequency of micronuclei (MN) were observed both in blood and in bone marrow cells in mice. 1,3-BD did not, however, increase the frequency of MN in either blood or bone marrow cells of rats at any of the tested concentrations.
将雌性CB6F1小鼠和雄性Wistar大鼠通过吸入暴露于不同浓度的1,3 - 丁二烯（1,3 - BD）。对这些动物的外周血红细胞和股骨骨髓细胞进行了微核试验。细胞染色采用传统的吖啶橙（AO）染色或使用AO包被载玻片进行活体染色。在小鼠的血液和骨髓细胞中均观察到微核（MN）频率呈剂量依赖性增加。然而，在任何测试浓度下，1,3 - BD均未增加大鼠血液或骨髓细胞中的MN频率。
