Shrestha P, Yang L T, Liu B L, Namba M, Qin C L, Isono K, Tsukitani K, Mori M
Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.
Anticancer Res. 1994 May-Jun;14(3A):825-36.
A total of 14 cases of clear cell carcinoma of salivary glands were evaluated by immunohistochemical methods using monoclonal antibodies to cytokeratin (K1.1 and K8.12), vimentin, S-100 alpha and beta subunits, neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), MAM-3 and MAM-6 antigens and proliferating cell nuclear antigen (PCNA), as well as polyclonal antibodies to lysozyme (Ly), lactoferrin (la) and Alpha-1-antichymotrypsin (alpha 1-Ach). Histopathologically, the carcinoma was characterized by round or polygonal tumor cells with cytoplasm that does not stain with hematoxylin and eosin, nuclei with little pleomorphism and few or no mitotic figures, and growing in solid sheets, small nests or cords with collagenous stroma. Cytokeratin KL1 and K8.12 was present in few tumor cells with almost negligible to strong reaction in all cases, vimentin in 6, GFAP in 5 cases with multiple-expression of cytokeratin K8.12, vimentin and GFAP in 5 cases. S-100 protein immunoreactivity was the most prominent feature with more intense reaction of S-100 beta than S-100 alpha subunit. NSE reactivity was seen in 6 cases. Ly, La, a1-ch, MAM-3 and MAM-6 antigens were localized in clear cells with various reaction intensities. The authors conclude that the clear tumor cells in clear cell carcinoma of salivary glands are not myoepithelial in origin but epithelial or neuroectodermal/neural crest in origin, showing ductal differentiation at the immunohistochemical level.
采用针对细胞角蛋白（K1.1和K8.12）、波形蛋白、S-100α和β亚基、神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）、MAM-3和MAM-6抗原以及增殖细胞核抗原（PCNA）的单克隆抗体，以及针对溶菌酶（Ly）、乳铁蛋白（la）和α1-抗糜蛋白酶（α1-Ach）的多克隆抗体，通过免疫组织化学方法对14例涎腺透明细胞癌进行了评估。组织病理学上，该癌的特征为圆形或多边形肿瘤细胞，其细胞质苏木精-伊红染色不着色，细胞核异形性小，有丝分裂象少见或无，呈实性片状、小巢状或条索状生长，伴有胶原性间质。细胞角蛋白KL1和K8.12在少数肿瘤细胞中存在，所有病例中反应几乎可忽略不计至强阳性；波形蛋白在6例中表达，GFAP在5例中表达，其中5例同时表达细胞角蛋白K8.12、波形蛋白和GFAP。S-100蛋白免疫反应性是最显著的特征，S-100β亚基的反应比S-100α亚基更强。NSE反应在6例中可见。Ly、La、α1-ch、MAM-3和MAM-6抗原定位于透明细胞，反应强度各异。作者得出结论，涎腺透明细胞癌中的透明肿瘤细胞并非起源于肌上皮，而是起源于上皮或神经外胚层/神经嵴，在免疫组织化学水平显示导管分化。
