• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Technical report. Video imaging of ethidium bromide-stained DNA gels with surface UV illumination.

作者信息

Solioz M

机构信息

University of Berne, Switzerland.

出版信息

Biotechniques. 1994 Jun;16(6):1130-3.

PMID:7521189
Abstract

We describe here the use of surface UV illumination to record ethidium bromide-stained DNA gels with a video camera. This mode of illumination allows the use of a standard video camera equipped with a red filter and results in a high signal strength. The assembly of a low-cost video system on this basis is described. It uses the public domain software called Image on a Macintosh computer and PostScript laser printer or a thermal printer to generate hard copies. The setup is sensitive enough to detect 500 pg of DNA on an ethidium bromide-stained DNA gel. The UV illumination method described here can also greatly improve the sensitivity of existing video recording equipment.

摘要

相似文献

1
Technical report. Video imaging of ethidium bromide-stained DNA gels with surface UV illumination.
Biotechniques. 1994 Jun;16(6):1130-3.
2
A video technique for the quantification of DNA in gels stained with ethidium bromide.一种用于对用溴化乙锭染色的凝胶中的DNA进行定量的视频技术。
Anal Biochem. 1995 Jul 1;228(2):336-42. doi: 10.1006/abio.1995.1360.
3
A desktop, low-cost video fluorometer for quantitation of macromolecules after gel electrophoresis.一种用于凝胶电泳后大分子定量分析的台式低成本视频荧光计。
Appl Theor Electrophor. 1995;4(4):175-7.
4
High resolution scanning of absorbing and fluorescent electrophoresis gels using video image analysis.使用视频图像分析对吸收性和荧光电泳凝胶进行高分辨率扫描。
Comput Appl Biosci. 1987 Nov;3(4):339-43.
5
Cost-effective gel documentation using a web-cam.使用网络摄像头进行具有成本效益的凝胶成像记录。
J Biochem Biophys Methods. 2001 Dec 4;50(1):91-5. doi: 10.1016/s0165-022x(01)00174-9.
6
A charge-coupled-device camera image analysis system for quantifying DNA distributions in agarose gels after pulsed-field gel electrophoresis.一种用于在脉冲场凝胶电泳后定量分析琼脂糖凝胶中DNA分布的电荷耦合器件相机图像分析系统。
Radiat Res. 1994 Oct;140(1):37-47.
7
DAPI, a simple sensitive alternative to ethidium bromide staining of DNA in agarose gels.4',6-二脒基-2-苯基吲哚（DAPI），一种用于琼脂糖凝胶中DNA溴化乙锭染色的简单灵敏替代方法。
Appl Theor Electrophor. 1993;3(5):253-5.
8
Factors affecting quantitation of DNA bands in gels using a charge-coupled device imaging system.
Electrophoresis. 2001 Mar;22(5):860-3. doi: 10.1002/1522-2683()22:5<860::AID-ELPS860>3.0.CO;2-D.
9
Zinc-imidazole positive: a new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity.
Electrophoresis. 1996 Jan;17(1):26-9. doi: 10.1002/elps.1150170105.
10
Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators.SYBR Gold核酸凝胶染色剂的特性：一种为配合300纳米紫外透射仪使用而优化的染料。
Anal Biochem. 1999 Mar 15;268(2):278-88. doi: 10.1006/abio.1998.3067.