精胺对J774.2巨噬细胞中一氧化氮合酶诱导的抑制作用：血清因子的需求
Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages: requirement of a serum factor.
作者信息
Szabó C, Southan G J, Wood E, Thiemermann C, Vane J R
机构信息
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London.
出版信息
Br J Pharmacol. 1994 Jun;112(2):355-6. doi: 10.1111/j.1476-5381.1994.tb13078.x.
Abstract
Polyamines are endogenous regulators of various cell functions. Nitric oxide (NO) is a cytostatic and cytotoxic free radical which is produced by the inducible NO synthase (iNOS) in immuno-stimulated macrophages. We tested whether spermine modulates the induction of iNOS in J774.2 macrophages. Stimulation of macrophages by bacterial lipopolysaccharide (LPS) or gamma-interferon increased the accumulation of nitrite in the culture medium. Spermine (10(-6) - 10(-4) M) inhibited nitrite production without causing cytotoxicity. This inhibition of NO formation by spermine was significantly reduced when it was given 6 h after LPS. Spermine did not inhibit nitrite accumulation when foetal calf serum was omitted from the tissue culture medium. Thus, spermine is an inhibitor of the induction of iNOS, and its inhibitory activity requires the presence of a serum factor.
摘要
多胺是多种细胞功能的内源性调节因子。一氧化氮（NO）是一种具有细胞生长抑制和细胞毒性作用的自由基，由免疫刺激的巨噬细胞中的诱导型一氧化氮合酶（iNOS）产生。我们测试了精胺是否调节J774.2巨噬细胞中iNOS的诱导。用细菌脂多糖（LPS）或γ-干扰素刺激巨噬细胞会增加培养基中亚硝酸盐的积累。精胺（10^(-6)-10^(-4)M）可抑制亚硝酸盐的产生而不引起细胞毒性。当在LPS刺激6小时后给予精胺时，其对NO形成的抑制作用显著降低。当组织培养基中省略胎牛血清时，精胺不会抑制亚硝酸盐的积累。因此，精胺是iNOS诱导的抑制剂，其抑制活性需要血清因子的存在。
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