Watanabe S, Kimura Y, Honda M, Sasaki J
Department of Anatomy, Okayama University Medical School, Japan.
J Electron Microsc (Tokyo). 1994 Apr;43(2):119-21.
A simple method for staining the superficial cells of mouse urinary bladder epithelium with horseradish peroxidase (HRP) is presented. HRP solution was instilled into the bladder lumen in vivo. After fixation with glutaraldehyde, the bladder was treated with Triton X-100 and then reacted with diaminobenzidine (DAB). Intense DAB reactions were detected throughout the whole of the cytoplasm of superficial cells by both light and electron microscopy. No reaction was observed in intermediate or basal epithelial cells. This simple method will be useful for the three-dimensional reconstruction of cell shape and identification of the stratification of transitional epithelial cell layers.
本文介绍了一种用辣根过氧化物酶（HRP）对小鼠膀胱上皮表层细胞进行染色的简单方法。将HRP溶液经体内滴注到膀胱腔内。用戊二醛固定后，膀胱用曲拉通X-100处理，然后与二氨基联苯胺（DAB）反应。通过光学显微镜和电子显微镜均检测到表层细胞的整个细胞质中出现强烈的DAB反应。中间层或基底上皮细胞未观察到反应。这种简单方法将有助于细胞形状的三维重建以及移行上皮细胞层分层的鉴定。
