Mager A, Masengo R, Mammerickx M, Letesson J J
Immunology Unit, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium.
J Gen Virol. 1994 Sep;75 ( Pt 9):2223-31. doi: 10.1099/0022-1317-75-9-2223.
Peripheral blood mononuclear cells (PBMCs) from bovine leukaemia virus (BLV)-seronegative cattle and from BLV-seropositive cows either with normal haematological values or persistent lymphocytosis were tested for their proliferative response to BLV antigens. Cells from only BLV-infected cattle with normal lymphocyte counts were stimulated to a detectable level by the fetal lamb kidney cell supernatant containing BLV antigens. Proliferation assays performed with the purified major core protein p24 indicated that this protein has to be processed through a chloroquine-sensitive compartment before being recognized by CD4+ T lymphocytes. Forty-one 15-mer overlapping peptides spanning the entire p24 sequence were synthesized and analysed for their stimulating potential. It appeared that two regions included T cell epitopes recognized by PBMCs from three of five animals tested. These regions were represented by amino acids 31 to 55 (PGSQVWIQTLRLAILQADPTPADLE) and 141 to 165 (AESYVEFVNRLQISLADNLPDGVPK). The possible implication of this cell-mediated immune response in BLV pathogenesis and vaccine development is discussed.
对来自牛白血病病毒（BLV）血清阴性牛以及具有正常血液学值或持续性淋巴细胞增多症的BLV血清阳性奶牛的外周血单个核细胞（PBMC）进行检测，以观察其对BLV抗原的增殖反应。只有来自淋巴细胞计数正常的BLV感染牛的细胞，被含有BLV抗原的胎羊肾细胞上清液刺激到可检测水平。用纯化的主要核心蛋白p24进行的增殖试验表明，该蛋白在被CD4⁺ T淋巴细胞识别之前，必须通过一个对氯喹敏感的区室进行加工处理。合成了覆盖整个p24序列的41个15肽重叠肽，并分析了它们的刺激潜力。结果显示，两个区域包含了来自所检测的五只动物中的三只动物的PBMC所识别的T细胞表位。这些区域由氨基酸31至55（PGSQVWIQTLRLAILQADPTPADLE）和141至165（AESYVEFVNRLQISLADNLPDGVPK）代表。本文讨论了这种细胞介导免疫反应在BLV发病机制和疫苗开发中的可能意义。
