Dube Syamalima, Abbott Lynn, Dube Dipak K, Dolcini Guillermina, Gutierrez Silvina, Ceriani Carolina, Juliarena Marcela, Ferrer Jorge, Perzova Raisa, Poiesz Bernard J
Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA.
Virol J. 2009 Aug 3;6:120. doi: 10.1186/1743-422X-6-120.
DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
从感染了阿根廷一头持续血清学检测不确定、病毒载量低的BLV感染的荷斯坦奶牛（编号41）外周血单个核细胞后在体内感染了BLV毒株的羔羊淋巴细胞中提取DNA。用一系列覆盖整个BLV前病毒DNA的重叠引物对DNA进行PCR扩增。扩增的BLV ARG 41 DNA被克隆、测序，并与包括来自阿根廷同一牛群的体内高复制毒株（BLV ARG 38）在内的其他BLV序列进行系统发育比较。讨论了BLV ARG 41推导蛋白的特征及其与逆转录病毒PTLV/BLV属其他成员的关系。
