间接免疫荧光显微镜技术在类鼻疽杆菌菌落鉴定中的应用。

Application of indirect immunofluorescence microscopy to colony identification of Pseudomonas pseudomallei.

作者信息

Naigowit P, Kurata T, Wangroongsub P, Petkanjanapong V, Kondo E, Kanai K

机构信息

National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

出版信息

Asian Pac J Allergy Immunol. 1993 Dec;11(2):149-54.

PMID:7521644

Abstract

Indirect immunofluorescence microscopy was used as a colony identification method of Pseudomonas pseudomallei isolates. The antisera against lipopolysaccharide and protein fractions of P. pseudomallei were prepared in guinea pigs and rabbits. With these antisera and fluorescence-labelled anti-guinea pig IgG and anti-rabbit IgG prepared in sheep (goat), indirect immunofluorescence microscopy was conducted on the colonies of P. pseudomallei and other species of bacteria. The overall results indicated that this method is efficient, rapid and specific for identification of P. pseudomallei colonies from clinical specimens.

摘要

间接免疫荧光显微镜技术被用作鉴定类鼻疽假单胞菌分离株菌落的方法。针对类鼻疽假单胞菌脂多糖和蛋白质组分的抗血清在豚鼠和兔子体内制备。使用这些抗血清以及在绵羊（山羊）体内制备的荧光标记抗豚鼠IgG和抗兔IgG，对类鼻疽假单胞菌及其他细菌种类的菌落进行间接免疫荧光显微镜检测。总体结果表明，该方法对于从临床标本中鉴定类鼻疽假单胞菌菌落高效、快速且具有特异性。