Stafford A N, Rider S H, Hopkin J M, Cookson W O, Monaco A P
Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Hum Mol Genet. 1994 May;3(5):779-85. doi: 10.1093/hmg/3.5.779.
An important locus for Atopy (familial asthma, hay fever and eczema) has been localized to the 11q12-q13 region with the minimum recombination fraction around the CD20 gene. We have constructed a 2.8 megabase (Mb) Yeast Artificial Chromosome (YAC) contig of the candidate region using 15 STSs. A total of seven genes have been mapped within this interval in the order cen-OSBP-TCN1-GIF-Fc epsilon RI beta-CD20-CD5-PGA-q(ter) and can be covered by a minimum of eight YAC clones. Contig integrity was assayed with fluorescence in-situ hybridization (FISH) and the mapping of YAC ends on somatic cell and radiation hybrid panels. A long range restriction map of the contig has been constructed to establish the order of and distance between loci. Two promising candidates for the atopy locus, the beta subunit of the high affinity immunoglobulin E receptor (Fc epsilon RI beta) and CD20, a molecule involved in B cell differentiation, have been placed within the contig.
一个与特应性（家族性哮喘、花粉症和湿疹）相关的重要基因座已被定位到11q12 - q13区域，最小重组率位于CD20基因附近。我们使用15个序列标签位点（STSs）构建了候选区域的一个2.8兆碱基（Mb）酵母人工染色体（YAC）重叠群。在这个区间内总共定位了7个基因，顺序为着丝粒 - OSBP - TCN1 - GIF - FcεRIβ - CD20 - CD5 - PGA - q（端粒），并且至少可以被8个YAC克隆覆盖。通过荧光原位杂交（FISH）以及YAC末端在体细胞和辐射杂种板上的定位来检测重叠群的完整性。已构建该重叠群的长程限制性图谱以确定基因座之间的顺序和距离。特应性基因座的两个有前景的候选基因，即高亲和力免疫球蛋白E受体（FcεRIβ）的β亚基和参与B细胞分化的分子CD20，已被定位在该重叠群内。
