Petrov T, Krukoff T L, Jhamandas J H
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Cell Tissue Res. 1994 Aug;277(2):289-95. doi: 10.1007/BF00327776.
Triple fluorescence labelling was employed to reveal the distribution of chemically identified neurons within the pontine laterodorsal tegmental nucleus and dorsal raphe nucleus which supply branching collateral input to the central nucleus of the amygdala and hypothalamic paraventricular nucleus. The chemical identity of neurons in the laterodorsal tegmental nucleus was revealed by immunocytochemical detection of choline-acetyltransferase or substance P; in the dorsal raphe nucleus, the chemical content of the neurons was revealed with antibody recognizing serotonin. The projections were defined by injections of two retrograde tracers, rhodamine- and fluorescein-labelled latex microspheres, in the central nucleus of the amygdala and paraventricular nucleus, respectively. Neurons projecting to both the central nucleus of the amygdala and the paraventricular nucleus were distributed primarily within the caudal extensions of the laterodorsal tegmental nucleus and dorsal raphe nucleus. Approximately 11% and 7% of the labelled cells in the laterodorsal tegmental nucleus and dorsal raphe nucleus projected via branching collaterals to the paraventricular nucleus and central nucleus of the amygdala. About half of these neurons in the laterodorsal tegmental nucleus were cholinergic, and one-third were substance-P-ergic; in the dorsal raphe nucleus, approximately half of the neurons containing both retrograde tracers were serotonergic. These results indicate that pontine neurons may simultaneously transmit signals to the central nucleus of the amygdala and paraventricular nucleus and that several different neuroactive substances are found in the neurons participating in these pathways. This coordinated signalling may lead to synchronized responses of the central nucleus of the amygdala and paraventricular nucleus for the maintenance of homeostasis.(ABSTRACT TRUNCATED AT 250 WORDS)
采用三重荧光标记法来揭示脑桥背外侧被盖核和中缝背核内化学性质已明确的神经元的分布情况，这些核向杏仁核中央核和下丘脑室旁核提供分支侧支输入。通过免疫细胞化学检测胆碱乙酰转移酶或P物质来揭示背外侧被盖核中神经元的化学性质；在中缝背核中，用识别5-羟色胺的抗体来揭示神经元的化学物质含量。通过分别向杏仁核中央核和室旁核注射两种逆行示踪剂（罗丹明和荧光素标记的乳胶微球）来确定投射。投射到杏仁核中央核和室旁核的神经元主要分布在背外侧被盖核和中缝背核的尾端延伸部分。背外侧被盖核和中缝背核中约11%和7%的标记细胞通过分支侧支投射到室旁核和杏仁核中央核。背外侧被盖核中约一半的这些神经元是胆碱能的，三分之一是P物质能的；在中缝背核中，约一半含有两种逆行示踪剂的神经元是5-羟色胺能的。这些结果表明脑桥神经元可能同时向杏仁核中央核和室旁核传递信号，并且在参与这些通路的神经元中发现了几种不同的神经活性物质。这种协调的信号传导可能导致杏仁核中央核和室旁核的同步反应以维持体内平衡。（摘要截短于250词）
