荧光差异显示：在自动DNA测序仪上进行的任意引物RT-PCR指纹分析。
Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer.
作者信息
Ito T, Kito K, Adati N, Mitsui Y, Hagiwara H, Sakaki Y
机构信息
Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
出版信息
FEBS Lett. 1994 Sep 5;351(2):231-6. doi: 10.1016/0014-5793(94)00867-1.
We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.
我们建立了用于“差异显示（DD）”的稳健、可靠的方案，差异显示是一种最初由Liang和Pardee于1992年开发的RNA指纹识别方法，该方法使用带有任意引物的逆转录聚合酶链反应（RT-PCR）。我们对方案进行了优化，以便能够在荧光DNA测序仪上进行可靠的差异显示分析，与使用放射性化合物的原始方法相比，这确保了高通量以及更高的操作安全性。这种“荧光差异显示（FDD）”技术将加速对差异表达以及多态性转录本的鉴定，以解决各种生物学问题。
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