Möhle R, Haas R, Hunstein W
Department of Internal Medicine V, University of Heidelberg, Germany.
J Hematother. 1993 Winter;2(4):483-9. doi: 10.1089/scd.1.1993.2.483.
We assessed the expression of the adhesion molecules leukocyte function antigen-1 (LFA-1, CD11a), intercellular adhesion molecule-1 (ICAM-1, CD54), homing-associated cell adhesion molecule (H-CAM, CD44), and c-kit (stem cell factor receptor) on the CD34+ progenitor population from the leukapheresis products of 23 patients (LP CD34+). For blood stem cell collection granulocyte colony-stimulating factor (G-CSF) or interleukin-3/granulocyte-macrophage colony-stimulating factor (IL-3/GM-CSF) was administered after cytotoxic chemotherapy. Furthermore, bone marrow- and blood-derived CD34+ progenitor cells from 6 normal volunteers (BM and PB CD34+) were analyzed. LFA-1 expression was higher on PB CD34+ (88.2 +/- 2.5%, mean +/- SEM) than on BM CD34+ (75.3 +/- 4.3%). Following cytokine administration, LFA-1 was expressed on only 59.7 +/- 3.7% of LP CD34+ at a low fluorescence intensity, suggesting that down-regulation of LFA-1 may facilitate the egress of cells from the bone marrow and prolong their circulation. In contrast, ICAM-1 was weakly positive on CD34+ cells from all sources. CD44 was expressed on the vast majority of CD34+ cells (> 95%) in all samples studied. The highest proportion of CD34+ cells costaining for c-kit was found in normal bone marrow (32.2 +/- 3.3%). In normal peripheral blood and after cytokine mobilization, fewer of the CD34+ cells weakly expressed c-kit (< 15%). The low percentage and level of c-kit expression may indicate that the majority of cytokine-mobilized CD34+ cells are lineage-committed progenitor cells, as reflected by the coexpression pattern for CD38, HLA-DR, and CD33.
我们评估了23例患者白细胞分离产物中CD34⁺祖细胞群体（LP CD34⁺）上黏附分子白细胞功能抗原-1（LFA-1，CD11a）、细胞间黏附分子-1（ICAM-1，CD54）、归巢相关细胞黏附分子（H-CAM，CD44）和c-kit（干细胞因子受体）的表达。对于造血干细胞采集，在细胞毒性化疗后给予粒细胞集落刺激因子（G-CSF）或白细胞介素-3/粒细胞-巨噬细胞集落刺激因子（IL-3/GM-CSF）。此外，分析了6名正常志愿者的骨髓和血液来源的CD34⁺祖细胞（BM和PB CD34⁺）。PB CD34⁺上LFA-1的表达（88.2±2.5%，平均值±标准误）高于BM CD34⁺（75.3±4.3%）。细胞因子给药后，LFA-1仅在59.7±3.7%的LP CD34⁺上以低荧光强度表达，这表明LFA-1的下调可能有助于细胞从骨髓中逸出并延长其循环时间。相比之下，ICAM-1在所有来源的CD34⁺细胞上呈弱阳性。在所有研究样本中，绝大多数CD34⁺细胞（>95%）表达CD44。c-kit共染色的CD34⁺细胞比例最高的是在正常骨髓中（32.2±3.3%）。在正常外周血和细胞因子动员后，较少的CD34⁺细胞弱表达c-kit（<15%）。c-kit表达的低百分比和水平可能表明，大多数细胞因子动员的CD34⁺细胞是定向祖细胞，这通过CD38、HLA-DR和CD33的共表达模式得以体现。
