Teofili L, Iovino M S, Di Mario A, Ortu La Barbera E, Pierelli L, Bussa S, Rumi C, Menichella G, Leone G
Department of Hematology, Sacro Cuore University, Roma, Italy.
Int J Artif Organs. 1993 Dec;16 Suppl 5:89-95.
Hemopoietic CD34+ progenitors were isolated by immunomagnetic method from normal bone marrow (BM) or from peripheral blood (PB) of patients with non-Hodgkin's lymphoma treated with chemotherapy and granulocyte colony-stimulating factor (GCSF). Aliquots were seeded in long-term cultures (LTC) on bone marrow-derived stromal layers; non-adherent and adherent clonogenic content of the cultures was assayed weekly. The final recovery and the clonogenic efficiency of the CD34+ cells were slightly higher in PB samples than in BM controls. In long term cultures PB cells sustained hemopoiesis as much as BM cells; at week 3 and 4 PB total mononuclear cells and CD34+ cells showed a non-adherent cell recovery higher than the respective BM controls. Furthermore, PB CD34+ cells were expanded in liquid culture in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) or G-CSF alone or combined with interleukin 3 (IL3), stem cell factor (SCF), interleukin 1 (IL1), interleukin 6 (IL6). The combination of GM-CSF, IL3, SCF, IL1 and IL6 produced the maximum increase of both mononuclear cells (30-fold) and granulocyte-macrophage colony forming units (CFU-GM) (4.6-fold) after 7 days of cultures; yet after 14 days a strong decrease of the CFU-GM occurred. These data suggest that G-CSF following chemotherapy mobilizes both early and committed hemopoietic progenitors.
采用免疫磁珠法从正常骨髓（BM）或接受化疗及粒细胞集落刺激因子（GCSF）治疗的非霍奇金淋巴瘤患者的外周血（PB）中分离造血CD34+祖细胞。将等分试样接种于骨髓来源的基质层上进行长期培养（LTC）；每周检测培养物中不贴壁和贴壁的克隆形成细胞含量。PB样本中CD34+细胞的最终回收率和克隆形成效率略高于BM对照。在长期培养中，PB细胞维持造血的能力与BM细胞相当；在第3周和第4周，PB总单核细胞和CD34+细胞的不贴壁细胞回收率高于各自的BM对照。此外，PB CD- +细胞在单独存在粒细胞-巨噬细胞集落刺激因子（GM-CSF）或G-CSF或与白细胞介素3（IL3）、干细胞因子（SCF）、白细胞介素1（IL1）、白细胞介素6（IL6）联合的液体培养中得以扩增。GM-CSF、IL3、SCF、IL1和IL6联合使用在培养7天后使单核细胞（30倍）和粒细胞-巨噬细胞集落形成单位（CFU-GM）（4.6倍）均出现最大程度的增加；然而在14天后CFU-GM出现显著下降。这些数据表明化疗后使用G-CSF可动员早期和定向造血祖细胞。
