Furuya K, Makimura N, Mitsui C, Ishikawa N, Tokuoka S, Imaizumi E, Mizumoto Y, Seki K, Nagata I, Ivell R
Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Japan.
Horm Res. 1994;41 Suppl 1:41-7. doi: 10.1159/000183942.
Recently, several reports have demonstrated the presence of oxytocin (OT) in the corpus luteum of mammalian species. However, the biological role of ovarian OT remains obscure. This study was performed to examine OT gene expression in cumulus cells of mice and humans, and in human corpus luteum, and the role of OT in early embryogenesis. OT gene and OT mRNA were analyzed by reverse transcription-polymerase chain reaction, with single-strand-conformation polymorphism and heteroduplex procedures. OT-treated in-vitro-fertilized mouse oocytes were cultured and the rate of blastocyst development estimated. An immunohistochemical study was also carried out to detect OT on the surface of the mouse oocytes.
最近，有几份报告证明了哺乳动物物种的黄体中存在催产素（OT）。然而，卵巢OT的生物学作用仍不清楚。本研究旨在检测小鼠和人类卵丘细胞以及人类黄体中的OT基因表达，以及OT在早期胚胎发生中的作用。采用逆转录-聚合酶链反应、单链构象多态性和异源双链分析方法对OT基因和OT mRNA进行分析。对经OT处理的体外受精小鼠卵母细胞进行培养，并评估囊胚发育率。还进行了免疫组织化学研究以检测小鼠卵母细胞表面的OT。
