Furuya K, Mizumoto Y, Makimura N, Mitsui C, Murakami M, Tokuoka S, Ishikawa N, Imaizumi E, Katayama E, Seki K
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama, Japan.
Horm Res. 1995;44 Suppl 2:47-9. doi: 10.1159/000184661.
Oxytocin (OT) has been detected in mammalian granulosa-luteal cells during the early stages. The purpose of this study was to explore gene expressions of OT and OT receptor (OTR) in human cumulus cells. Cumulus cells enclosing a mature oocyte were obtained from 6 women undergoing clinical in vitro fertilization and embryo transfer programs. OT and OTR gene expressions were investigated by employing reverse transcription polymerase chain reaction and reverse transcription polymerase chain reaction/single-strand conformation polymorphism methods. OT gene expression in the cumulus cells was positive in 5 women and weakly positive in the remaining patient. The structure of OT mRNA in the cumulus cells was equivalent to that in human hypothalamus. OTR gene expression was also observed in the cumulus cells. This study is the first to describe the simultaneous expression of both OT and OTR genes in human cumulus cells. It is suggested that local OT plays some important roles in fertility through modification of the micro-environment around the oocyte.
在早期阶段，已在哺乳动物的颗粒黄体细胞中检测到催产素（OT）。本研究的目的是探讨人卵丘细胞中OT和OT受体（OTR）的基因表达。从6名接受临床体外受精和胚胎移植程序的女性中获取包裹成熟卵母细胞的卵丘细胞。采用逆转录聚合酶链反应和逆转录聚合酶链反应/单链构象多态性方法研究OT和OTR基因表达。5名女性的卵丘细胞中OT基因表达为阳性，其余患者为弱阳性。卵丘细胞中OT mRNA的结构与人类下丘脑的结构相同。在卵丘细胞中也观察到OTR基因表达。本研究首次描述了人卵丘细胞中OT和OTR基因的同时表达。提示局部OT通过改变卵母细胞周围的微环境在生育中发挥重要作用。
