Klosen P, Van den Bosch de Aguilar P
Laboratory of Cell Biology, Catholic University of Louvain, Louvain-la-Neuve, Belgium.
J Neurocytol. 1994 May;23(5):297-311. doi: 10.1007/BF01188498.
We and other researchers have previously described the presence of axon-specific phosphorylated neurofilament epitopes in the cell bodies of three neuronal types in the rat: bipolar septofimbrial neurons and the large light A-type cells in the dorsal root ganglia and the mesencephalic nucleus of the Vth nerve. This spontaneous presence of phosphorylated neurofilaments at the level of the perikaryon contrasts with the induced appearance of these epitopes in axotomized neurons. We have undertaken a study of this phenomenon in rat, mouse, gerbil, rabbit, pig and chicken to analyse its species distribution. Phosphorylated neurofilament positive perikarya could be detected in the dorsal root ganglia and mesencephalic nucleus of the Vth nerve in all analysed species. Although this labelling has been shown to be specific for A-type cells in rat, in pig small cells were preferentially labelled, whereas the largest cells were mostly completely devoid of label. In the septofimbrial nucleus, phosphorylated neurofilament positive perikarya were seen in rat, mouse, gerbil and rabbit. In the pig, only a phosphatase-insensitive neurofilament antibody labelled these neurons. In the chicken, the labelling was completely absent. These observations establish the widespread species distribution of perikaryal phosphorylated neurofilament epitopes in the dorsal root ganglia and mesencephalic nucleus of the Vth nerve. In the septofimbrial nucleus however, this phenomenon seems to be restricted to rodents and lagomorphs. We discuss possible explanations for these cytoskeletal singularities in dorsal root ganglia, the mesencephalic nucleus of the Vth nerve and septofimbrial neurons.
我们和其他研究人员之前曾描述过，在大鼠的三种神经元类型的细胞体中存在轴突特异性磷酸化神经丝表位：双极隔缘束状神经元、背根神经节中的大型亮色 A 型细胞以及三叉神经中脑核。这种磷酸化神经丝在核周体水平的自发存在与轴突切断的神经元中这些表位的诱导出现形成对比。我们对大鼠、小鼠、沙鼠、兔子、猪和鸡的这一现象进行了研究，以分析其物种分布情况。在所有分析的物种中，均可在背根神经节和三叉神经中脑核中检测到磷酸化神经丝阳性核周体。尽管这种标记已被证明对大鼠的 A 型细胞具有特异性，但在猪中，小细胞优先被标记，而最大的细胞大多完全没有标记。在隔缘束状核中，在大鼠、小鼠、沙鼠和兔子中可见磷酸化神经丝阳性核周体。在猪中，只有一种对磷酸酶不敏感的神经丝抗体标记了这些神经元。在鸡中，完全没有这种标记。这些观察结果表明，核周体磷酸化神经丝表位在背根神经节和三叉神经中脑核中具有广泛的物种分布。然而，在隔缘束状核中，这种现象似乎仅限于啮齿动物和兔形目动物。我们讨论了背根神经节、三叉神经中脑核和隔缘束状神经元中这些细胞骨架异常现象的可能解释。
