Holford L C, Case P, Lawson S N
Department of Physiology, Medical School, Bristol, UK.
J Neurocytol. 1994 Sep;23(9):577-89. doi: 10.1007/BF01262058.
Immunocytochemical studies on lumbar dorsal root ganglia obtained at routine postmortem 24-36 h after death were carried out, and neuronal cross-sectional areas measured. The subjects were elderly (76-81 years), of both sexes, had died from heart attack or haemorrhage, and had no clinical evidence of clinical neuropathy or of disease known to be associated with neuropathy. The data were consistent between ganglia from the three subjects. There were striking similarities with data from other species. Two populations of cell profiles with overlapping size distributions were distinguished with an anti-neurofilament antibody, neurofilament-rich (45% of cell profiles) with a large mean area and neurofilament-poor with a smaller mean area. Anti-substance P and anti-peripherin antibodies both labelled a population with a small mean area, with extensive co-localization between them. There were also some differences between these human dorsal root ganglia and dorsal root ganglia from some other species. More neuronal profiles were labelled for substance P in humans (44%) than in rat (20%). More neuronal profiles were labelled for SSEA4 (stage specific embryonic antigen 4) in human (40.5%) than in rat dorsal root ganglia (10%), and the SSEA4-positive profiles were relatively smaller in human than in rat. No selective accumulation of lipofusin in profiles of large cells was apparent. This study also shows that quantitative morphometric analysis of immunocytochemically labelled dorsal root ganglion neuronal profiles can be carried out successfully on human sensory ganglia obtained at post-mortem. This is the first demonstration of the two main subgroups of dorsal root ganglia neurones with neurofilament-rich and poor somata in human tissue. The size distributions of neurons with neurofilament, substance P and peripherin are consistent with these neuronal populations having similar functional properties to those described in other species. From the known sensory and fibre loss with aging, it is speculated that the loss of some large diameter neurones with myelinated fibres and low mechanical thresholds, might account for the high percentage of neurones expressing substance P.
对死亡后24 - 36小时常规尸检获取的腰段背根神经节进行了免疫细胞化学研究，并测量了神经元的横截面积。受试者为老年人（76 - 81岁），男女均有，死于心脏病发作或出血，且无临床神经病变证据或已知与神经病变相关疾病的临床证据。来自三名受试者神经节的数据一致。与其他物种的数据有显著相似性。用抗神经丝抗体可区分出两个大小分布重叠的细胞轮廓群体，富含神经丝的（占细胞轮廓的45%）平均面积大，神经丝少的平均面积小。抗P物质抗体和抗外周蛋白抗体均标记了一个平均面积小的群体，它们之间有广泛的共定位。这些人类背根神经节与其他一些物种的背根神经节之间也存在一些差异。人类中标记P物质的神经元轮廓（44%）比大鼠（20%）多。人类中标记阶段特异性胚胎抗原4（SSEA4）的神经元轮廓（40.5%）比大鼠背根神经节（10%）多，且人类中SSEA4阳性轮廓相对大鼠较小。未观察到脂褐素在大细胞轮廓中有选择性积累。本研究还表明，对尸检获得的人类感觉神经节进行免疫细胞化学标记的背根神经节神经元轮廓的定量形态学分析可以成功进行。这是首次在人体组织中证明背根神经节神经元存在富含神经丝和神经丝少的两个主要亚组。具有神经丝、P物质和外周蛋白的神经元大小分布与这些神经元群体具有与其他物种中描述的相似功能特性一致。从已知的衰老导致的感觉和纤维丧失推测，一些具有髓鞘纤维和低机械阈值的大直径神经元的丧失，可能解释了表达P物质的神经元的高比例。
