Dellacorte C
Department of Biology, University of Southern California, Los Angeles 90089-2520.
Tissue Cell. 1994 Aug;26(4):613-9. doi: 10.1016/0040-8166(94)90013-2.
Standard procedures for isolating nucleic acids from specialized tissues such as the mucus-containing tissues found in many marine organisms are, in many cases, not effective, resulting in isolates contaminated with polysaccharides that encumber subsequent analysis. A method is described for isolating nucleic acids from the sea anemone Condylactis gigantea (Cnidaria: Anthozoa) using the compound hexadecyltrimethyl ammonium bromide (CTAB). This substance has historically been effective in producing digestible chromosomal DNA from a variety of polysaccharide-enriched sources. In the presence of CTAB, DNA can be isolated and extracted from Condylactis gigantea and is suitable for digestion with restriction endonucleases. With a minor modification, RNA can also be extracted and used to obtain mRNA. The technique is useful for Cnidarian tissues and may be appropriate for a variety of other marine invertebrates and algae.
在许多情况下，从特殊组织（如许多海洋生物中含黏液的组织）中分离核酸的标准程序并不有效，导致分离出的核酸被多糖污染，从而妨碍后续分析。本文描述了一种使用十六烷基三甲基溴化铵（CTAB）从海葵巨大拟柱头虫（刺胞动物门：珊瑚纲）中分离核酸的方法。从历史上看，这种物质在从各种富含多糖的来源中产生可消化的染色体DNA方面一直很有效。在CTAB存在的情况下，可以从巨大拟柱头虫中分离和提取DNA，并且该DNA适用于用限制性内切酶进行消化。稍作修改后，也可以提取RNA并用于获得mRNA。该技术对刺胞动物组织有用，也可能适用于各种其他海洋无脊椎动物和藻类。
