Kniep B, Cinek T, Angelisová P, Horejsí V
GBF, Immunobiology Group, Braunschweig, Germany.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):1069-75. doi: 10.1006/bbrc.1994.2291.
The major glycolipid co-immunopurifying with the glycosylphosphatidylinositol-anchored leucocyte surface glycoprotein CD59 from detergent lysates of human T cell lines HPB ALL, Jurkat and myeloid line HL-60 was identified as the glycosphingolipid GM3. Monoclonal antibodies to GM3 immunoprecipitated the same large detergent-resistant, protein-tyrosine kinase containing "GPI-complexes" as antibodies to several GPI-anchored proteins. Therefore GM3 is another component of these large membrane complexes potentially involved in signalling through GPI-anchored receptors or through some glycolipids.
从人T细胞系HPB ALL、Jurkat以及髓系细胞系HL-60的去污剂裂解物中，与糖基磷脂酰肌醇锚定的白细胞表面糖蛋白CD59共同免疫纯化的主要糖脂被鉴定为神经节苷脂GM3。针对GM3的单克隆抗体与针对几种糖基磷脂酰肌醇锚定蛋白的抗体一样，能免疫沉淀相同的大型抗去污剂、含蛋白酪氨酸激酶的“GPI复合物”。因此，GM3是这些大型膜复合物的另一个组分，可能参与通过糖基磷脂酰肌醇锚定受体或某些糖脂进行的信号传导。
