Bibbo M, Xiao J, Christen R, Fitzpatrick B, Galera-Davidson H, Bartels P H, Minimo C
Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-5244.
Anal Quant Cytol Histol. 1994 Jun;16(3):183-8.
An inexpensive workstation is being developed to assist pathologists in diagnosing routine hematoxylin and eosin-stained slides. A linear discriminant model was applied to karyometric features of prostate lesions, and a grade according to Mostofi was determined from the discriminant values. Twenty cases, five of hyperplasia and five each of carcinoma Mostofi grades I, II and III, for a total of 600 nuclei, were selected to train the model. Computer graphic filters were constructed from the discriminant values. Each segmented nucleus has a colored frame (the graphic filter) displayed around it. The color, determined from discriminant values and correlated with the grades, ranges from green for hyperplasia, yellow for low grade, orange for medium grade and red for high grade. An additional 20 cases, 5 of hyperplasia and 5 each of the Mostofi grades, for a total of 538 nuclei, were selected to test the graphic filter. Ninety-six percent of the hyperplasia nuclei were framed in green, 84% of low grade nuclei were framed in yellow, 90% of medium grade nuclei were framed in orange, and 89% of high grade nuclei were framed in red. These results indicate the potential of the color graphic filter to show the pathologist immediate and accurate visual information about the grade of a nucleus. This method may help with the difficult diagnosis of borderline lesions and may help in making the diagnosis from scanty biopsy material.
正在开发一种低成本的工作站，以协助病理学家诊断常规苏木精和伊红染色的玻片。将线性判别模型应用于前列腺病变的核测量特征，并根据判别值确定莫斯托菲分级。选择20个病例，其中增生5例，莫斯托菲I级、II级和III级癌各5例，共600个细胞核，用于训练模型。根据判别值构建计算机图形滤波器。每个分割的细胞核周围都显示有一个彩色边框（图形滤波器）。颜色由判别值确定并与分级相关，从增生的绿色到低级别黄色、中级别的橙色和高级别的红色。另外选择20个病例，其中增生5例，莫斯托菲各级别各5例，共538个细胞核，用于测试图形滤波器。96%的增生细胞核被绿色边框包围，84%的低级别细胞核被黄色边框包围，90%的中级细胞核被橙色边框包围，89%的高级细胞核被红色边框包围。这些结果表明彩色图形滤波器有潜力向病理学家显示关于细胞核级别的即时准确视觉信息。这种方法可能有助于诊断临界病变，并可能有助于从少量活检材料中做出诊断。
