Markova D, Donev R, Patriotis C, Djondjurov L
Department of Cell Proliferation, Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
DNA Cell Biol. 1994 Sep;13(9):941-51. doi: 10.1089/dna.1994.13.941.
DNA of the attachment sites of Friend erythroleukemia cells, isolated according to the conventional procedure, represents short, nuclease-resistant fragments with sizes below 400 bp, belonging to the class of mouse satellite. A number of experiments have indicated that their unusual resistance is due to complexing with RNA. By various approaches, it was confirmed that similar fragments might be recovered from total DNA following extensive digestion with DNase I. In situ hybridizations revealed further that at mitosis the sequences of the attachment sites are located at the centromeric/telomeric regions of the chromosomes, while at interphase they are redistributed into 9-13 well-defined clusters spread throughout the entire nuclear area. Parallel biochemical and electronmicroscopic studies have clarified, moreover, that the all three compartments of the matrix harbor such sequences. Thus, it appears that the attachment sites described function only at interphase, anchoring the both ends of each interphase chromosome to the matrix structures.
按照常规程序分离得到的弗氏红白血病细胞附着位点的DNA，呈现出短的、耐核酸酶的片段，大小在400 bp以下，属于小鼠卫星DNA类别。许多实验表明，它们不同寻常的抗性是由于与RNA形成复合物。通过各种方法证实，用DNase I进行广泛消化后，可从总DNA中回收类似片段。原位杂交进一步显示，在有丝分裂时，附着位点的序列位于染色体的着丝粒/端粒区域，而在间期它们重新分布到9 - 13个明确的簇中，散布在整个核区域。此外，平行的生化和电子显微镜研究表明，核基质的所有三个部分都含有此类序列。因此，所描述的附着位点似乎仅在间期起作用，将每个间期染色体的两端锚定到基质结构上。
