Kristoffersen E K, Ulvestad E
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
APMIS. 1994 Jul;102(7):509-13. doi: 10.1111/j.1699-0463.1994.tb05198.x.
Nitric oxide synthase is demonstrated immunohistochemically in the cytosol, on granules, and on syncytiotrophoblasts membranes. The enzyme is also detected on placental villous stroma cells, and on endothelial cells. The histochemical staining method NADPH-diaphorase stains the syncytiotrophoblasts intensely, and stroma cells more weakly. Membranes of syncytiotrophoblasts immobilized on nitrocellulose paper are also stained by NADPH-diaphorase, and by antisera to nitric oxide synthase. Oxidases of sex steroid synthesis do, however, influence placental trophoblasts and there are discrepancies in the staining pattern of endothelial cells. Caution should therefore be exercised when using NADPH-diaphorase as a staining method for nitric oxide synthase in placenta.
免疫组织化学显示，一氧化氮合酶存在于胞质溶胶、颗粒以及合体滋养层细胞膜上。该酶在胎盘绒毛基质细胞和内皮细胞中也能检测到。组织化学染色方法NADPH-黄递酶能强烈地染色合体滋养层细胞，而对基质细胞的染色较弱。固定在硝酸纤维素纸上的合体滋养层细胞膜也能被NADPH-黄递酶和一氧化氮合酶抗血清染色。然而，性类固醇合成的氧化酶确实会影响胎盘滋养层细胞，并且内皮细胞的染色模式存在差异。因此，在使用NADPH-黄递酶作为胎盘一氧化氮合酶的染色方法时应谨慎。
