Wörl J, Wiesand M, Mayer B, Greskötter K R, Neuhuber W L
Institute of Anatomy, University of Erlangen-Nürnberg, Germany.
Histochemistry. 1994 Nov;102(5):353-64. doi: 10.1007/BF00268906.
In this study, we wished to clarify the distribution and co-localization of nitric oxide synthase and NA-DPH-diaphorase (NADPH-d) in nerve cells, nerve fibres and parenchymal cells in exocrine and endocrine pancreas, and to assess the influence of fixation on the staining pattern obtained. For this purpose, we applied nitric oxide synthase immunocytochemistry and NADPH-d histochemistry to rat and human pancreas under different fixation conditions. Antibodies to neuronal and endothelial nitric oxide synthase were similarly applied. We found complete co-localization of neuronal nitric oxide synthase and NADPH-d in ganglion cells, and in nerve fibres around acini, excretory ducts, blood vessels and in islets of Langerhans of rat and human pancreas. Immunoreactivity for endothelial nitric oxide synthase was co-localized with NADPH-d in endothelial cells. However, in NADPH-d reactive islet and ductal epithelial cells we could detect neither brain nor endothelial nitric oxide synthase immunoreactivity with any fixation protocol applied. There were marked differences in NADPH-d staining of both neurons and parenchymal cells under different fixation conditions. These results indicate the existence of different types of NADPH-d, which are associated or not associated with nitric oxide synthase(s), and which are differently influenced by various fixation procedures in rat and human pancreas.
在本研究中，我们希望阐明一氧化氮合酶和还原型辅酶Ⅱ黄递酶（NADPH-d）在神经细胞、神经纤维以及外分泌和内分泌胰腺的实质细胞中的分布与共定位情况，并评估固定对所获染色模式的影响。为此，我们在不同固定条件下，对大鼠和人类胰腺应用一氧化氮合酶免疫细胞化学和NADPH-d组织化学方法。同样应用了针对神经元型和内皮型一氧化氮合酶的抗体。我们发现，在大鼠和人类胰腺的神经节细胞、腺泡周围的神经纤维、排泄管、血管以及胰岛中，神经元型一氧化氮合酶与NADPH-d完全共定位。内皮型一氧化氮合酶的免疫反应性与NADPH-d在内皮细胞中共定位。然而，在NADPH-d反应性胰岛和导管上皮细胞中，采用任何固定方案，我们均未检测到脑型或内皮型一氧化氮合酶的免疫反应性。在不同固定条件下，神经元和实质细胞的NADPH-d染色存在显著差异。这些结果表明，存在不同类型的NADPH-d，它们与一氧化氮合酶相关或不相关，并且在大鼠和人类胰腺中受各种固定程序的影响不同。
