α-胰蛋白酶抑制剂重链H1和H3基因的串联方向

Tandem orientation of the inter-alpha-trypsin inhibitor heavy chain H1 and H3 genes.

作者信息

Diarra-Mehrpour M, Bourguignon J, Sarafan N, Bost F, Sesboüé R, Muschio-Bonnet F, Martin J P

机构信息

Laboratoire de Physiopathologie et Génétique Rénale et Pulmonaire, Institut National de la Santé et de la Recherche Médicale, INSERM Unité 295, Faculté de Médicine de Rouen, France.

出版信息

Biochim Biophys Acta. 1994 Oct 18;1219(2):551-4. doi: 10.1016/0167-4781(94)90087-6.

DOI:10.1016/0167-4781(94)90087-6

PMID:7522574

Abstract

The inter-alpha-trypsin inhibitor H1 (ITIH1) and inter-alpha-trypsin inhibitor H3 (ITIH3) genes have both previously been mapped to chromosomes 3 and 14 in the human and mouse, respectively. We now present evidence that these genes are physically linked. By using cDNA probes, a recombinant DNA phage has been isolated from a bacteriophage DNA library, which contains sequences flanking the 5' end of the ITIH3 gene and the 3' end of the ITIH1 gene. Restriction endonuclease mapping, PCR analysis and DNA sequence determination of the recombinant phage and comparison to genomic DNA revealed that the genes are in tandem, 2721 base pairs apart, with the ITIH1 gene to the 5' side of the ITIH3 gene. Their respective transcriptional units are thus on the same strand of DNA and most probably arose in evolution as the consequence of a duplication of a common ancestral gene.

摘要

α-胰蛋白酶抑制剂H1（ITIH1）基因和α-胰蛋白酶抑制剂H3（ITIH3）基因先前分别被定位到人类染色体3和小鼠染色体14上。我们现在提供证据表明这些基因在物理上是连锁的。通过使用cDNA探针，从噬菌体DNA文库中分离出一个重组DNA噬菌体，它包含ITIH3基因5'端侧翼序列和ITIH1基因3'端侧翼序列。对重组噬菌体进行限制性内切酶图谱分析、PCR分析和DNA序列测定，并与基因组DNA进行比较，结果显示这两个基因串联排列，相距2721个碱基对，ITIH1基因位于ITIH3基因的5'侧。因此，它们各自的转录单元位于DNA的同一条链上，很可能是在进化过程中由一个共同祖先基因的复制产生的。