Ingram R S, Scott R W, Tilghman S M
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4694-8. doi: 10.1073/pnas.78.8.4694.
The urine alpha-fetoprotein (AFP) and serum albumin genes most probably arose in evolution as the consequence of a duplication of a common ancestral gene. They have both been previously mapped to chromosome 5 in the mouse. We now have evidence that these genes are closely linked. By using a unique copy DNA probe derived from previously cloned AFP 5' flanking DNA, a recombinant DNA phage has been isolated, from a bacteriophage DNA library, that contains sequences flanking the 5' end of the AFP gene and the 3' end of the albumin gene. Restriction endonuclease mapping and DNA sequence determination of the recombinant phage and comparison to total genomic DNA confirmed that the genes are in tandem, 13.5 kilobase pairs apart, with the albumin gene to the 5' side of the AFP gene. Thus, they are transcribed from the same strand of DNA.
尿甲胎蛋白（AFP）基因和血清白蛋白基因很可能是在进化过程中由一个共同祖先基因的复制产生的。它们之前都已被定位到小鼠的5号染色体上。我们现在有证据表明这些基因紧密连锁。通过使用从先前克隆的AFP 5'侧翼DNA衍生而来的独特单拷贝DNA探针，从噬菌体DNA文库中分离出了一个重组DNA噬菌体，它包含AFP基因5'端和白蛋白基因3'端的侧翼序列。对重组噬菌体进行限制性内切酶图谱分析和DNA序列测定，并与总基因组DNA进行比较，证实这两个基因是串联排列的，相距13.5千碱基对，白蛋白基因位于AFP基因的5'侧。因此，它们从DNA的同一条链转录而来。
