Hughes K J, Everiss K D, Harkey C W, Peterson K M
Department of Microbiology and Immunology, Louisiana State University Medical Center-Shreveport 71130.
Gene. 1994 Oct 11;148(1):97-100. doi: 10.1016/0378-1119(94)90240-2.
The toxin-coregulated pilus (TCP)-encoding gene cluster (tcp) specifies a type-IV pilus that is a major colonization determinant of Vibrio cholerae. We have identified a gene 200 bp upstream from the tcp cluster that requires ToxR for expression. We have designated this gene tagD (ToxR-activated gene) and have shown that tagD is encoded on a 600-nt transcript. The deduced tagD product is a 164-amino-acid polypeptide (20 kDa). Interestingly, TagD shares a high degree of similarity to a protein of Streptococcus sanguis 12 that is thought to play a role in fimbriae synthesis or assembly. The high degree of similarity between tagD and the Ss 12 protein provides preliminary evidence that tagD represents an additional member of the tcp cluster.
毒素协同调节菌毛（TCP）编码基因簇（tcp）决定了一种IV型菌毛，它是霍乱弧菌主要的定植决定因素。我们在tcp基因簇上游200 bp处鉴定出一个基因，其表达需要ToxR。我们将该基因命名为tagD（ToxR激活基因），并表明tagD由一个600 nt的转录本编码。推导的tagD产物是一个164个氨基酸的多肽（20 kDa）。有趣的是，TagD与血链球菌12的一种蛋白质高度相似，后者被认为在菌毛合成或组装中起作用。tagD与Ss 12蛋白之间的高度相似性提供了初步证据，表明tagD是tcp基因簇的另一个成员。
