Stevens M G, Olsen S C, Cheville N F
Brucellosis Research Unit, National Animal Disease Center, Ames, Iowa 50010.
Infect Immun. 1994 Oct;62(10):4646-9. doi: 10.1128/iai.62.10.4646-4649.1994.
Lymphocyte proliferation in response to proteins from the Brucella abortus strain 2308 (S2308) and the lipopolysaccharide (LPS) O-antigen-deficient mutant of S2308, strain RB51 (SRB51), was measured in S2308-infected cattle following abortion. Supramammary and superficial cervical lymph node lymphocytes from infected cattle proliferated most when incubated with 27- to 18-kDa proteins of S2308 or SRB51. Proteins of SRB51, which contained no LPS O antigens, induced lymphocyte proliferation similar to that induced by S2308 proteins, which contained LPS O antigens. These results indicate that 27- to 18-kDa proteins, but not LPS O antigens, of S2308 and SRB51 are immunodominant in S2308-infected cattle as assessed by lymphocyte proliferation assays.
在流产后的2308株布鲁氏菌（S2308）感染牛中，检测了其淋巴细胞对来自流产布鲁氏菌2308株（S2308）和S2308的脂多糖（LPS）O抗原缺陷突变株RB51（SRB51）的蛋白的增殖反应。感染牛的乳房上淋巴结和颈浅淋巴结淋巴细胞在与S2308或SRB51的27至18 kDa蛋白一起孵育时增殖最为明显。不含LPS O抗原的SRB51蛋白诱导的淋巴细胞增殖与含LPS O抗原的S2308蛋白诱导的相似。这些结果表明，通过淋巴细胞增殖试验评估，S2308和SRB51的27至18 kDa蛋白而非LPS O抗原在S2308感染牛中具有免疫优势。
